Real Time PCR

1、Real Time PCR Product Overview

Real Time PCR is an analytical instrument that monitors the DNA amplification process in real time using fluorescent signals, enabling precise quantification of nucleic acids.The Real Time PCR is a single-channel, 8-well fluorescence quantitative PCR instrument that combines high performance, intelligence, and exceptional reliability. It integrates a precise optical detection system, efficient and stable temperature control technology, and a highly flexible and convenient user experience.  It is dedicated to providing a fast, accurate, and reliable integrated solution for molecular diagnostics, life science research, pathogen detection, and gene expression analysis.

2、Real Time PCR Product Advantages

1. Intelligent Operating System, Complete Control Over Experiments

A. Program Updates and Presets: Supports online updates of experimental programs and includes a wealth of built-in preset experimental program templates. Users can also customize and save frequently used templates for quick one-click startup, significantly improving operational efficiency.

B. Simple Operation: Both the touchscreen interface and computer software are designed to be intuitive and easy to understand, allowing new users to quickly get started. Pre-installed common program templates further simplify the operation process.

2. Precise and Stable Temperature Control and Optical Core

A. Long-lasting and Precise Temperature Control System: Uses imported high-quality TEC semiconductor cooling modules for precise temperature control (±0.1°C) and excellent temperature uniformity between wells (≤±0.3°C). The system boasts excellent stability, minimal performance degradation over long-term use, and a calibration cycle of several years, significantly reducing maintenance costs and operational downtime.

B. Maintenance-Free and Sensitive Optical System: Equipped with a single independent LED light source and a single independent photodiode detector, using an ultra-long-life imported light source.  It is maintenance-free throughout its design life, requiring no additional calibration or replacement, ensuring long-term consistency and high sensitivity of detection results.

C. Excellent Data Quality: High experimental linearity (R²≥0.999) and excellent repeatability (low CV value between wells) ensure accurate and reliable quantitative results and high data confidence.

3. Efficient and Convenient Data and Output Management

A. Multiple Data Export Methods: Supports direct copying via USB storage devices or data transfer to local servers or the cloud via Wi-Fi wireless network, providing convenience and flexibility to meet various laboratory data management habits.

B. Rich Report Export and Printing: Supports generating and exporting experimental reports in various formats (such as PDF, Excel). The instrument is compatible with USB thermal printers, Bluetooth wireless printers, and conventional A4 printers, allowing for one-click export and printing of professional reports directly from the instrument or via a computer, facilitating result archiving and instant sharing.

4. Fast, Efficient, and Durable

A. Fast Heating and Cooling Speed: The optimized temperature control system has high-speed heating and cooling capabilities, significantly shortening single-cycle and overall detection times, increasing experimental throughput.

B. Ultra-Long Service Life: Core components are made of industrial-grade durable materials and design, ensuring stable and reliable operation of the entire machine, providing users with a long-term return on investment.

3、Real Time PCR Application Scenarios

Basic scientific research, pathogen detection, meat product adulteration detection, genetically modified organism (GMO) detection, food safety testing, drug development and rational drug use, gene expression, water body monitoring, pine wood nematode detection, pet testing, and livestock disease detection.

4、Real Time PCR Technical Specifications

1. Features a high-grade aluminum alloy sample holder with excellent thermal conductivity, corrosion resistance, and oxidation resistance, along with Peltier heating and temperature control, and a comprehensive sealing design. Neither sample solution nor condensed water will leak into the Peltier temperature control element or other electronic components below the sample holder, effectively extending the instrument's lifespan.

2. Employs a high-precision PT1000 temperature sensor, achieving a temperature control accuracy of 0.01℃, ensuring accurate temperature control.

3. Sample throughput: 0.2ml single tube, 0.2ml 8-tube strip.

4. Open reagent and consumables system: Compatible with reagents from any brand, suitable for standard 8-tube strips or 0.2ml PCR single tubes.

5. Utilizes a blue, high-intensity, broad-spectrum, long-life LED solid-state light source, ensuring strong light intensity across the entire spectral detection range, improving detection sensitivity. It also generates no heat, has a long lifespan, and is maintenance-free for life.

6. High-sensitivity detector: Uses a high-sensitivity photomultiplier tube imported from Japan, resulting in higher detection sensitivity.

7. No optical path correction required: The scanning system ensures that the signal excitation and detection optical paths are completely consistent for each well, eliminating the need for optical path correction. ★

8. Number of channels: Single channel configuration.

9. Channel crosstalk: Uses high-precision narrow-band filters to greatly reduce channel crosstalk, and the software can also correct crosstalk parameters. 10. Detection Sensitivity: Capable of detecting single-copy DNA templates and clearly distinguishing between 1000 and 2000 copies of plasmid DNA.

11. Linear Detection Range: 10 orders of magnitude ★

12. Supports common fluorescent dyes and probe detection methods, such as FAM and SYBR Green I.

13. Module Temperature Range: 4-99℃

14. Operating Mode: Built-in PCR operating system on the system screen ★

15. Operating Noise: Silent technology, maximum operating noise does not exceed 60dB during operation.

16. Operation and Analysis Software

16.1 Allows defining sample wells after running the program, saving experimental time.

16.2 Allows opening other experimental data results for viewing and analysis while the program is running.

16.3 The software automatically calculates the baseline range and sets the threshold line, providing users with reliable and accurate data results.

16.4 Experimental program settings and detection results can be directly printed as an experimental report, supporting various file formats and data styles such as CSV and PDF. ★

16.5 Results from the same batch of experiments can be grouped for independent data analysis.

16.6 Provides both Chinese and English software interfaces. ★

Real Time PCR Instrument Technical Specifications

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