Bursaphelenchus xylophilus detector

1.Purpose of Bursaphelenchus xylophilus detector instrument

Bursaphelenchus xylophilus detector can be applied to forests, parks, orchards, urban greening and other fields to help managers detect and control pine wood nematodes in time.Bursaphelenchus xylophilus detector mainly includes the processes of isolation of pine wood nematode, DNA extraction, fluorescence quantitative PCR detection and detection.It can quickly complete the detection work of pine wood nematode, automatically display the detection results and print the detection report without manual interpretation.The accuracy rate is greater than 99%, which greatly improves the efficiency of pine wood nematode detection work.It is equipped with a field quick inspection system that can be portable and mobile.The operation provides convenience for the detection of pine wood nematode in the field.The pine wood nematode automated molecular detection system is widely used in forestry bureaus, forest control stations, quarantine bureaus, customs, universities, scientific research institutions and other units.

2.Instrument features of Bursaphelenchus xylophilus detector

1.System detection accuracy is high.

2.The intelligent pine wood nematode DNA rapid detection system has a built-in automatic pine wood nematode DNA detection program, which does not require complicated program settings and runs automatically with one click.It can not only detect nematode separation liquid, but also directly detect pine wood chips without separating nematodes.

3.The system detection operation is easy to operate, time-saving and efficient, and is suitable for forestry and forestry defense departments at all levels.The entire process including sample processing, DNA extraction, on-machine detection and result interpretation can be completed in as little as 60 minutes.

4.Using amplified fluorescence technology, laboratory testing and identification needs can be completed through the system host.

5.Small size, light weight, easy to carry.Easily meet the needs of out-of-town experiments.

6.Built-in 10-inch high-definition capacitive screen PDA, touch screen operation, easy and fast.

7.Marlow high-quality Peltier refrigeration film, combined with the German high-end PT1000 temperature sensor and the temperature control mode of the electrical resistance heating compensation edge, has a maximum heating speed of 7℃ and a maximum cooling speed of 5℃, which greatly shortens the experimental time.

8.3s fast lighting mode for the entire board to ensure the consistency of hole positions in experimental results.

9.Simple and intuitive software guidance makes it easy to start detection experiments.

3.Technical parameters of Bursaphelenchus xylophilus detector

Sample capacity: 48x0.2ml, supports 8 strips

Applicable consumables: common transparent PCR consumables, 8x0.2ml tubes, 0.2ml single tube

Reaction system: 5-120ul reaction mode system

Heating/cooling module: high-performance imported semiconductor thermoelectric module

Temperature control range: 4°C-99°C

Hot cover temperature: 30℃-108℃ (default 105℃, hot cover temperature is adjustable)

Heating rate: 3.5℃/s(MAX)

Cooling rate: 2.5℃/s(MAX)

Temperature control accuracy: ±0.1°C

Temperature Uniformity: ±0.25°C

Temperature accuracy: ±0.1℃

Number of temperature control areas: multiple points (2 points)

Number of gradients: 0

Gradient temperature range: None

Number of gradient holes: None

Excitation light source: high-power LED (maintenance-free, long life)

Excitation light wavelength range: 400-700nm

Detection components: Imported high-sensitivity photoelectric sensor

Excitation and detection propagation medium: high temperature resistant professional light guide fiber

Number of detection channels: 4 channels as standard

Applicable dyes and probes:

First channel: 470/520 FAM, SYBR Green

Second channel: 530/570 HEX, JOE, VIC

Third channel: 580/610 ROX, CY3.5, Texas-Red

Fourth channel: 630/670 CY5

Software function: multiplex qPCR data analysis function, can detect 4 detection channels at the same time

amplification curve,

Standard curve quantification,

melting curve,

Support HRM detection

Data export: Experimental results can be directly exported in the form of graphics and tables; detailed data report content includes experimental conditions, data coordinates and tables, and data analysis parameters.

Data format: XLS, PDF, DOCX

Noise: <45dB

Screen size: 10 inches (HD)

Touch screen: capacitive

External USB: supports data import and export

Hot lid: automatic pressure adjustment

4.Bursaphelenchus xylophilus detector configuration list

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